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Ectopic study of calcium phosphate cement seeded with pBMP‐2 modified canine bMSCs mediated by a non‐viral PEI derivative

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Ectopic study of calcium phosphate cement seeded with pBMP‐2 modified canine bMSCs mediated by a non‐viral PEI derivative

Auteurs : Kaige Lü [République populaire de Chine] ; Deliang Zeng [République populaire de Chine] ; Wenjie Zhang [République populaire de Chine] ; Lunguo Xia [République populaire de Chine] ; Ling Xu [République populaire de Chine] ; Xinquan Jiang [République populaire de Chine] ; Fuqiang Zhang [République populaire de Chine]

Source :

Cell Biology International [ 1065-6995 ] ; 2012-02.

RBID : ISTEX:25B38F449AF379B96596AA93C3DEE5DE7765DC78

Descripteurs français

Wicri :
- topic : Biomatériau.

English descriptors

Abstract

We have evaluated the ectopic new bone formation effects of CPC (calcium phosphate cement) seeded with pBMP‐2 (plasmids containing bone morphogenetic protein‐2 gene) transfected canine bMSCs (bone marrow stromal cells) mediated by a non‐viral PEI (polyethylenimine) derivative (GenEscort™ II) in nude mice. Canine bMSCs were transfected with pBMP‐2 or pEGFP (plasmids containing enhanced green fluorescent protein gene) mediated by GenEscort™ II in vitro, and the osteoblastic differentiation was explored by ALP (alkaline phosphatase) staining, ARS (alizarin red S) staining and RT—qPCR (real‐time quantitative PCR) analysis. Ectopic bone formation effects of CPC/pBMP‐2 transfected bMSCs were evaluated and compared with CPC/pEGFP transfected bMSCs or CPC/untransfected bMSCs through histological, histomorphological and immunohistochemical analysis 8 and 12 weeks post‐operation in nude mice. Transfection efficiency was up ∼35% as demonstrated by EGFP (enhanced green fluorescent protein) expression. ALP and ARS staining were stronger with pBMP‐2 gene transfection, and mRNA expression of BMP‐2 (bone morphogenetic protein‐2), Col 1 (collagen 1) and OCN (osteocalcin) in pBMP‐2 group was significantly up‐regulated at 6 and 9 days. Significantly higher NBV (new bone volume) was achieved in pBMP‐2 group than in the control groups at 8 and 12 weeks (P<0.05). In addition, immunohistochemical analysis indicated higher OCN expression in pBMP‐2 group (P<0.01). We conclude that CPC seeded with pBMP‐2 transfected bMSCs mediated by GenEscort™ II could enhance ectopic new bone formation in nude mice, suggesting that GenEscort™ II mediated pBMP‐2 gene transfer is an effective non‐viral method and CPC is a suitable scaffold for gene enhanced bone tissue engineering.

Url:

https://api.istex.fr/document/25B38F449AF379B96596AA93C3DEE5DE7765DC78/fulltext/pdf

DOI: 10.1042/CBI20100848

Affiliations:

République populaire de Chine

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkaline phosphatase</term>
<term>Biomaterials</term>
<term>Bmscs</term>
<term>Bmscs transfected</term>
<term>Bone formation</term>
<term>Bone marrow</term>
<term>Bone marrow stromal cells</term>
<term>Bone regeneration</term>
<term>Bone tissue</term>
<term>Bone tissue engineering</term>
<term>Bone volume</term>
<term>Calcium phosphate cement</term>
<term>Canine</term>
<term>Canine bmscs</term>
<term>Cell biol</term>
<term>Cell proliferation</term>
<term>Culture medium</term>
<term>Culture plates</term>
<term>Derivative</term>
<term>Ectopic</term>
<term>Ectopic bone formation</term>
<term>Ectopic study bmscs</term>
<term>Gene</term>
<term>Gene therapy</term>
<term>Gene transfection</term>
<term>Gene transfer</term>
<term>Genescorttm</term>
<term>Grant numbers</term>
<term>Image analysis</term>
<term>Immunocytochemistry staining</term>
<term>Immunohistochemical</term>
<term>Implant</term>
<term>Jiang</term>
<term>Journal compilation</term>
<term>Mineral phase</term>
<term>Ninth hospital</term>
<term>Nude mice</term>
<term>Osteogenic</term>
<term>Osteogenic differentiation</term>
<term>Pegfp</term>
<term>Pegfp group</term>
<term>Plasmid</term>
<term>Portland press</term>
<term>Primary cells</term>
<term>Protein gene</term>
<term>Regeneration</term>
<term>Scaffold</term>
<term>Shanghai</term>
<term>Shanghai jiao tong university</term>
<term>Stromal</term>
<term>Tissue regeneration</term>
<term>Transfected</term>
<term>Transfected bmscs</term>
<term>Transfection</term>
<term>Transfection efficiency</term>
<term>Untransfected</term>
<term>Untransfected bmscs</term>
<term>Untransfected cells</term>
<term>Untransfected control cells</term>
<term>Untransfected group</term>
<term>Zhang</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Alkaline phosphatase</term>
<term>Biomaterials</term>
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<term>Bone formation</term>
<term>Bone marrow</term>
<term>Bone marrow stromal cells</term>
<term>Bone regeneration</term>
<term>Bone tissue</term>
<term>Bone tissue engineering</term>
<term>Bone volume</term>
<term>Calcium phosphate cement</term>
<term>Canine</term>
<term>Canine bmscs</term>
<term>Cell biol</term>
<term>Cell proliferation</term>
<term>Culture medium</term>
<term>Culture plates</term>
<term>Derivative</term>
<term>Ectopic</term>
<term>Ectopic bone formation</term>
<term>Ectopic study bmscs</term>
<term>Gene</term>
<term>Gene therapy</term>
<term>Gene transfection</term>
<term>Gene transfer</term>
<term>Genescorttm</term>
<term>Grant numbers</term>
<term>Image analysis</term>
<term>Immunocytochemistry staining</term>
<term>Immunohistochemical</term>
<term>Implant</term>
<term>Jiang</term>
<term>Journal compilation</term>
<term>Mineral phase</term>
<term>Ninth hospital</term>
<term>Nude mice</term>
<term>Osteogenic</term>
<term>Osteogenic differentiation</term>
<term>Pegfp</term>
<term>Pegfp group</term>
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<term>Portland press</term>
<term>Primary cells</term>
<term>Protein gene</term>
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<term>Scaffold</term>
<term>Shanghai</term>
<term>Shanghai jiao tong university</term>
<term>Stromal</term>
<term>Tissue regeneration</term>
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<term>Untransfected</term>
<term>Untransfected bmscs</term>
<term>Untransfected cells</term>
<term>Untransfected control cells</term>
<term>Untransfected group</term>
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<front><div type="abstract" xml:lang="en">We have evaluated the ectopic new bone formation effects of CPC (calcium phosphate cement) seeded with pBMP‐2 (plasmids containing bone morphogenetic protein‐2 gene) transfected canine bMSCs (bone marrow stromal cells) mediated by a non‐viral PEI (polyethylenimine) derivative (GenEscort™ II) in nude mice. Canine bMSCs were transfected with pBMP‐2 or pEGFP (plasmids containing enhanced green fluorescent protein gene) mediated by GenEscort™ II in vitro, and the osteoblastic differentiation was explored by ALP (alkaline phosphatase) staining, ARS (alizarin red S) staining and RT—qPCR (real‐time quantitative PCR) analysis. Ectopic bone formation effects of CPC/pBMP‐2 transfected bMSCs were evaluated and compared with CPC/pEGFP transfected bMSCs or CPC/untransfected bMSCs through histological, histomorphological and immunohistochemical analysis 8 and 12 weeks post‐operation in nude mice. Transfection efficiency was up ∼35% as demonstrated by EGFP (enhanced green fluorescent protein) expression. ALP and ARS staining were stronger with pBMP‐2 gene transfection, and mRNA expression of BMP‐2 (bone morphogenetic protein‐2), Col 1 (collagen 1) and OCN (osteocalcin) in pBMP‐2 group was significantly up‐regulated at 6 and 9 days. Significantly higher NBV (new bone volume) was achieved in pBMP‐2 group than in the control groups at 8 and 12 weeks (P<0.05). In addition, immunohistochemical analysis indicated higher OCN expression in pBMP‐2 group (P<0.01). We conclude that CPC seeded with pBMP‐2 transfected bMSCs mediated by GenEscort™ II could enhance ectopic new bone formation in nude mice, suggesting that GenEscort™ II mediated pBMP‐2 gene transfer is an effective non‐viral method and CPC is a suitable scaffold for gene enhanced bone tissue engineering.</div>
</front>
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<name sortKey="Xia, Lunguo" sort="Xia, Lunguo" uniqKey="Xia L" first="Lunguo" last="Xia">Lunguo Xia</name>
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<name sortKey="Zeng, Deliang" sort="Zeng, Deliang" uniqKey="Zeng D" first="Deliang" last="Zeng">Deliang Zeng</name>
<name sortKey="Zeng, Deliang" sort="Zeng, Deliang" uniqKey="Zeng D" first="Deliang" last="Zeng">Deliang Zeng</name>
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<name sortKey="Zhang, Fuqiang" sort="Zhang, Fuqiang" uniqKey="Zhang F" first="Fuqiang" last="Zhang">Fuqiang Zhang</name>
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Ectopic study of calcium phosphate cement seeded with pBMP‐2 modified canine bMSCs mediated by a non‐viral PEI derivative

Ectopic study of calcium phosphate cement seeded with pBMP‐2 modified canine bMSCs mediated by a non‐viral PEI derivative

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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